polyclonal mouse anti-fmrp antibody  (Abnova)

Journal: Frontiers in Aging Neuroscience

Article Title: Reduced Levels of the Synaptic Functional Regulator FMRP in Dentate Gyrus of the Aging Sprague-Dawley Rat

doi: 10.3389/fnagi.2017.00384

Figure Lengend Snippet: Comparison of FMRP levels between aging and young rats based on proteomics, immunoblotting and immunofluorescence (IF) microscopy. (A) The protein abundance levels of FMRP in young and aging rats obtained from a quantitative proteomic study (left) and immunoblot analysis (right). (B) Distribution of FMRP immunofluorescence analyzed in the hippocampal subareas of young and aging rats. Fluorescence images represent 10×, 20×, and 100× magnification of DG with FMRP labeled cells and neuropil in young and aging rats. Graphs represent percentage (%) of FMRP immunoreactivity in the granular layer (GL) and hilar (H) subareas of the DG area, as well as percentage (%) of total FMRP immunoreactivity throughout the whole DG. The differences were evaluated using Student’s t -test ( p < 0.05). Values are expressed as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: For detection of FMRP protein the membrane was incubated in 3% non-fat milk, 1x TBS, 0.1% Tween-20 with 1:1000 polyclonal mouse anti-FMRP antibody (MAB2160, Millipore) overnight at 4°C followed by incubation for 1 h at room temperature in 1:10000 HRP-conjugated anti-mouse secondary antibody (ab6728, Abcam).

Techniques: Comparison, Western Blot, Immunofluorescence, Microscopy, Quantitative Proteomics, Fluorescence, Labeling

Journal: Frontiers in Aging Neuroscience

Article Title: Reduced Levels of the Synaptic Functional Regulator FMRP in Dentate Gyrus of the Aging Sprague-Dawley Rat

doi: 10.3389/fnagi.2017.00384

Figure Lengend Snippet: Functional enrichment analysis and FMRP (FMR1)-associated interaction network of significantly changed proteins in aging rats. (A) The GO categories of the most regulated biological processes and molecular functions associated with significantly changed proteins in the aging group. The GO enrichment analysis (Benjamini–Hochberg p -Values < 0.01) was performed using ClueGO-Cytoscape platform ( ; ). (B) The protein interaction network of significantly changed proteins centered around FMRP retrieved from IPA analysis (QIAGEN). Green color represents downregulated proteins and red color represents upregulated proteins in the aging group. The protein nodes are connected based on RNA/DNA – interactions (directional lines) or protein – protein interactions (simple lines). Autoregulation is represented by loops. The gene abbreviations are given in the text and Supplementary Table .

Article Snippet: For detection of FMRP protein the membrane was incubated in 3% non-fat milk, 1x TBS, 0.1% Tween-20 with 1:1000 polyclonal mouse anti-FMRP antibody (MAB2160, Millipore) overnight at 4°C followed by incubation for 1 h at room temperature in 1:10000 HRP-conjugated anti-mouse secondary antibody (ab6728, Abcam).

Techniques: Functional Assay, Protein-Protein interactions